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Enzyme reagent

Q:How long can the reagent be kept?

When kept at a temperature below 0゚C, the reagent powder can last for two years. When it is reconstituted into solution, it can be kept at a temperature below 0゚C for 180 days. Bromine water does not have a storage period but it loses utility once the color has faded.

Q:If there is no distilled water when preparing the reagent, is there another alternative?

If there is no distilled water, you can use commercial purified water instead. Do not use mineral water, RO water, etc.

Q:In the enzyme activity test, if the enzyme activity in the first cuvette is below 0.40 da/min, should the enzyme powder be added directly or is there another way available?

Adjustment of the enzyme activity only needs to be done when the cuvette is opened for the first time (if too much distilled water is added to result in over dilution, the only remedy is to add enzyme powder directly) and start with the control group directly for subsequent tests. There is no need to test the enzyme activity every single time.

Q:Is it required to keep re-packaged enzyme in ice and test it each time it is removed and thawed for tests? Will it affect the enzyme activity when tests are done at room temperature?

(1) It is not required to keep re-packaged enzyme in ice after it is thawed.

(2) The activity of enzyme will be affected when it stays in a high-temperature environment for too long. It is recommended to conduct tests in an air-conditioned room.

Q:What is the % of the alcohol used during extraction?

The 95% alcohol is used during extraction.